Customized protein,
at any quantity!
Whether you are interested in microgram level amounts to test your design for Proof of Concept, or want to scale up for broader testing, our team is ready to help you use our Nicotiana benthamiana based system to produce your recombinant protein.
Speed
Go from concept to purified protein in around one month empowering rapid iteration and data-driven refinement, giving you the edge.
Safe
Our ΔXF Nicotiana benthamiana lines yield proteins with human-compatible glycosylation, ensuring functionality comparable to mammalian expression systems.
Scalable
From Proof of Concept to cGMP production, we are eager to help your project by producing whatever quantity of protein needed.
Protein Designs Expressed
Ready To Be Your One Stop Shop!
What would that look like?
Stage 1: Proof of Concept
Goal: Demonstrate the expression and basic activity of your target protein in a plant-based system, with the option to compare and select the best candidate from 2–5 constructs for further development.
Key Activities:
- Codon optimization & vector construction
- Transient expression in N. benthamiana
- Small-scale purification
- Basic functional assay
- Parallel expression & analysis of multiple candidates for yield/activity comparison
Deliverables:
- Vector map(s) & sequences
- Yield data for each candidate
- SDS-PAGE / Western blot
- Functional assay results
- Comparative table with recommendations for lead selection
- Technical report
Stage 2: Process Optimization & Research-Grade Production
Goal: Improve yield, purity, and reproducibility while supplying research-grade protein suitable for preclinical work.
Key Activities:
- Optimize expression conditions & harvest timing
- Scale-up in greenhouse/plant factory
- Purification process development
- Analytical method development
Deliverables:
- Process development report
- Analytical data (purity, MW, glycosylation, endotoxin)
- Stability data
- Research-grade protein + CoA
Stage 3: Preclinical / GLP Toxicity Supply
Goal: Produce GLP-compliant material for toxicology and efficacy studies.
Key Activities:
- Pilot-scale controlled production
- Purification under GLP conditions
- Stability testing- Supply to GLP tox CRO
Deliverables:
- GLP-compliant batch CoA
- Batch record
- Stability report
- Material for toxicity studies
Stage 4A: GMP Phase 1 Supply
Goal: GMP production of drug substance/product for first-in-human trial.
Key Activities:
- Tech transfer to GMP facility
- GMP batch production
- Fill/finish
- GMP release testing
- Stability program start
Deliverables:
- GMP batch CoA
- GMP batch records
- Validation reports for CMC phase 1
- Initial stability data
- Material for clinical trial phase 1
Stage 4B: Regulatory Support
Goal: Prepare CMC & plant-specific documentation for IND/CTA
Key Activities:
- Compile CMC section
- Respond to agency queries
Deliverables:
- CMC module for IND/CTA
- Risk assessment report
- Regulatory Q&A support